BSc(Hons I), PhD, UNSW
Phone: +61 (0) 2 9385 1892
Fax: +61 (0)2 9385 3950
E-mail: m.larance@garvan.org.au

Role & Interests

My project is a collaboration between the BMSF and the Garvan Institute of Medical Research, which focuses on the proteins associated with intracellular transport vesicles with cells. Proteins associated with these vesicles are responsible for their creation from a donor compartment, targeted movement around the cell, and fusion with an acceptor compartment. The number of proteins needed to perform all these complex functions is quite large so a comprehensive proteomic strategy was employed. In order to identify these proteins we have used a 2D-LC-MS/MS approach to derive peptide fragmentation data, and database searching software such as Sequest and Mascot. This allows us to view how the proteins associated with transport vesicles change over time and under different external stimuli. Thus we can better understand the processes that govern intracellular vesicle transport with a global view rather than a narrow focus.