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The research project requires the proteomiic analysis of affinity pull-downs of subcellular fractions at the rate of 5 samples per week for
40 weeks in years 2 and 3 with an estimated 8 hours instrument time per 5 sample. The base cost for student operated access to mass spectrometry
at the BMSF is $86/hour to which the university contributes $50 (UNSW projects) and NSW Government Funding tied to NCRIS contributes $11. A balance of $25 is requested
from the ARC.
If space permits add further specific explanation of why mass spectrometry is necessary, for example: "Tandem mass spectrometry combined with
liquid chromatography is at present the most effective method of confident protein identification and the elucidation of post-translational
modification of proteins."
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